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KMID : 0545120100200020340
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 2 p.340 ~ p.345
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Production of 1,3-dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112
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Hu Zhong-Ce
Liu Zhi-Qiang
Zheng Yu-Guo
Shen Yin-Chu
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Abstract
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The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Gluconobacter oxydans ZJB09112 in shake flask and bubble column bioreactor. After fermentation in the optimized medium (g/L: yeast extract 5, glycerol 2.5, mannitol 22.5, K2HPO4 0.5, KH2PO4 0.5, MgSO4?7H2O 0.1, CaCO3 2.0, pH 5.0), when five times of glycerol feeding were made, 161.9 ± 5.9 g/l of DHA was attained at 88.7 ± 3.2% conversion rate of glycerol to DHA.
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KEYWORD
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1,3-dihydroxyacetone, Gluconobacter oxydans, glycerol, fed-batch fermentation, bubble column bioreactor
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